In this study, the effect of anthocyanic extracts of strawberry (Fragaria x ananassa Duch.) variety Jacona on the in vitro growth of Staphylococcus aureus associated to bovine mastitis was evaluated. Sensitivity tests were carried out on the anthocyanic extracts by disk difussion method (10 µg/disk-100 µg/disk), using the antibiotic Dicloxacillin® as positive control (50 µg/mL, 50 µL/disk) and percentages of inhibition of bacterial growth were determined. Anthocyanic extracts managed to inhibit the bacterial growth of the strain ATCC 27543 and the isolated STA28 of S. aureus up to 54% and 40%, respectively. The results showed the antimicrobial potential of anthocyanic extracts of strawberry against S. aureus associated to bovine mastitis.