Fast determination of glucosamine in pharmaceutical formulations by high performance liquid chromatography without pre-column derivatization
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Keywords

Glucosamine
pharmaceutical formulations
reversed phase liquid chromatography.

Abstract

The goal of this work was to achieve fast determination of glucosamine in pharmaceutical formulations by high performance liquid chromatography. Commercial tablets were dissolved in water and after filtration, 6 μL were injected to Luna C18 column (250 mm × 4.6 mm, 5μm). Diode Array Detector (DAD) was set at 193 nm. Using sodium perchlorate (50 mM, pH 6.5): acetonitrile (99:1, v/v) at 0.8 mL min-1 as a mobile phase, glucosamine eluted with the retention time 2.834 min ± 0.003 min and the peak purity in real sample was 998.9 ± 3.3. The detection limits evaluated for peak height and peak area measurements were 0.05 μg mL-1 and 0.02 µg mL-1, respectively. The between-day precision relative standard deviation (RSD) evaluated at two concentration levels was always below 1%.

https://doi.org/10.15174/au.2014.717
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