Análisis espectroscópico y efecto antifúngico de nanopartículas de plata sintetizadas con extracto acuoso de lúpulo Humulus lupulus L.
Publicado 2026-09-23
Derechos de autor 2026 Ulin Antobelli Basilio-Cortes, Alexis Alejandro Salazar-Navarro, Dagoberto Durán-Hernández, José Gregorio Joya-Dávila, Henry López-López, Lourdes González Salitre

Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-SinDerivadas 4.0.
Cómo citar
Resumen
La síntesis verde de nanopartículas de plata (AgNPs) es un área de oportunidad rápida y económica. En este estudio se analizó la síntesis de nanopartículas de sulfato de plata (AgSNPs) y nanopartículas de nitrato de plata (AgNNPs) utilizando extracto acuoso de lúpulo. Las AgNPs obtenidas fueron caracterizadas mediante métodos instrumentales espectroscópicos Se evaluó la actividad antifúngica contra Fusarium oxysporum y Alternaria solani. La presencia de AgNPs se evidenció por Uv-Vis en rangos de 200 nm a 350 nm. En FTIR se observaron picos entre 1000 cm⁻¹ a 1200 cm⁻¹ relacionados con la vibración de estiramiento del doble enlace C–C y C–O. El tamaño de partícula mostró comportamientos bimodales. En potencial ζ, las AgNPs presentaron valores entre 13.79 mV a -57.53 mV. Se presento actividad antifúngica con una disminución en los halos de crecimientos. Estas AgNPs sintetizadas con extracto de lúpulo puede ser una alternativa versátil antifúngica.
Referencias
- Alabrahim, O. A. A., Abdeldayem, A. M., & Azzazy, H. M. E. S. (2025). Green synthesis of metallic nanoparticles using Pistacia species: improved stability and biological activities. Nanoscale Advances, 7(18), 5449-5478. https://doi.org/10.1039/d5na00644a
- Anandalakshmi, K., Venugobal, J., & Ramasamy, V. (2016). Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity. Applied Nanoscience, 6, 399-408. https://doi.org/10.1007/s13204-015-0449-z
- Basilio-Cortes, U. A., Ramírez-Rodrigues, M. M., Ramírez-Rodrigues, M. A., González-Mendoza, D., Tzintzun-Camacho, O., Durán-Hernández, D., & González-Salitre, L. (2024). Phytochemical, spectroscopic analysis and antifungal activity on bell peppers of hydrothermal bioactive metabolites of Humulus lupulus L. extracts. Natural Product Research, 39(24), 7054-7065. https://doi.org/10.1080/14786419.2024.2405010
- Carotenuto, G., Palomba, M., Cristino, L., Di Grazia, M. A., De Nicola, S., & Nicolais, F. (2013). Preparation and characterization of antimicrobial silver/polystyrene nanocomposites. En G. Carotenuto & L. Nicolais (eds.), Nanocomposites: In Situ Synthesis of Polymer‐Embedded Nanostructures (pp. 195-207). Wiley. https://doi.org/10.1002/9781118742655.ch9
- Das, P., Dutta, T., Manna, S., Loganathan, S., & Basak, P. (2022). Facile green synthesis of non-genotoxic, non-hemolytic organometallic silver nanoparticles using extract of crushed, wasted, and spent Humulus lupulus (hops): characterization, anti-bacterial, and anti-cancer studies. Environmental Research, 204, 111962. https://doi.org/10.1016/j.envres.2021.111962
- Deka, K., Nongbet, R. D., Das, K., Saikia, P., Kaur, S., Talukder, A., & Thakuria, B. (2025). Understanding the mechanism underlying the green synthesis of metallic nanoparticles using plant extract(s) with special reference to silver, gold, copper and zinc oxide nanoparticles. Hybrid Advances, 9, 100399. https://doi.org/10.1016/j.hybadv.2025.100399
- Elmusa, F., Aygun, A., Gulbagca, F., Seyrankaya, A., Göl, F., Yenikaya, C., & Sen, F. (2021). Investigation of the antibacterial properties of silver nanoparticles synthesized using Abelmoschus esculentus extract and their ceramic applications. International Journal of Environmental Science and Technology, 18(4), 849-860. https://doi.org/10.1007/s13762-020-02883-x
- González-Salitre, L., González-Olivares, L. G., & Basilio-Cortes, U. A. (2023). Humulus lupulus L. a potential precursor to human health: high hops craft beer. Food Chemistry, 405, 134959. https://doi.org/10.1016/j.foodchem.2022.134959
- Górka, K., & Kubiński, K. (2023). Antifungal activity against human and plant mycopathogens, and green synthesis of silver nanoparticles exhibiting such activity. Applied Sciences, 14(1), 115. https://doi.org/10.3390/app14010115
- Hamelian, M., Zangeneh, M. M., Amisama, A., Varmira, K., & Veisi, H. (2018). Green synthesis of silver nanoparticles using Thymus kotschyanus extract and evaluation of their antioxidant, antibacterial and cytotoxic effects. Applied Organometallic Chemistry, 32(9), e4458. https://doi.org/10.1002/aoc.4458
- Jain, A. K., & Thareja, S. (2019). In vitro and in vivo characterization of pharmaceutical nanocarriers used for drug delivery. Artificial Cells, Nanomedicine, and Biotechnology, 47(1), 524-539. https://doi.org/10.1080/21691401.2018.1561457
- Jeevanandam, J., Barhoum, A., Chan, Y. S., Dufresne, A., & Danquah, M. K. (2018). Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations. Beilstein Journal of Nanotechnology, 9(1), 1050-1074. https://doi.org/10.3762/bjnano.9.98
- Khan, S., Zahoor, M., Khan, R. S., Ikram, M., & Islam, N. U. (2023). The impact of silver nanoparticles on the growth of plants: the agriculture applications. Heliyon, 9(6), e16928. https://doi.org/10.1016/j.heliyon.2023.e16928
- Kumar, M., Saini, R. V., Gupta, M., & Singh, R. (2025). Green synthesis of silver nanoparticle (Cha-AgNPs) using Chenopodium album extract and evaluation of their antifungal potential against pathogenic fungi. Biomass Conversion and Biorefinery, 15, 8955-8966. https://doi.org/10.1007/s13399-024-05721-z
- Kyaw, K., Harada, A., Ichimaru, H., Kawagoe, T., Yahiro, K., Morimura, S., Ono, K., Tsutsuki, H., Sawa, T., & Niidome, T. (2017). Silver nanoparticles as potential antibiofilm agents against human pathogenic bacteria. Chemistry Letters, 46(4), 594-596. https://doi.org/10.1246/cl.161198
- Le Ouay, B., & Stellacci, F. (2015). Antibacterial activity of silver nanoparticles: a surface science insight. Nanotoday, 10(3), 339-354. https://doi.org/10.1016/j.nantod.2015.04.002
- Malik, M. A., Wani, A. H., Bhat, M. Y., Siddiqui, S., Alamri, S. A. M., & Alrumman, S. A. (2024). Fungal-mediated synthesis of silver nanoparticles: a novel strategy for plant disease management. Frontiers in Microbiology, 15, 1399331. https://doi.org/10.3389/fmicb.2024.1399331
- Prasher, P., Singh, M., & Mudila, H. (2018). Green synthesis of silver nanoparticles and their antifungal properties. BioNanoScience, 8, 254-263. https://doi.org/10.1007/s12668-017-0481-4
- Rodrigues, T., Fontes, P., Silva, P. I., & Campos, P. (2021). A new perspective of a well-recognized raw material: phenolic content, antioxidant and antimicrobial activities and α-and β-acids profile of Brazilian hop (Humulus lupulus L.) extracts. LWT, 141, 110905. https://doi.org/10.1016/j.lwt.2021.110905
- Romero-Urbina, D. G., Lara, H. H., Velázquez-Salazar, J. J., Arellano-Jiménez, M. J., Larios, E., Srinivasan, A., López-Ribot, J. L., & Yacamán, M. J. (2015). Ultrastructural changes in methicillin-resistant Staphylococcus aureus induced by positively charged silver nanoparticles. Beilstein Journal of Nanotechnology, 6(1), 2396-2405. https://doi.org/10.3762/bjnano.6.246
- Titus, D., Samuel, E. J. J., & Roopan, S. M. (2019). Nanoparticle characterization techniques. In Green synthesis, characterization and applications of nanoparticles (pp. 303-319). Elsevier.
- Sharma, R., Dhillon, A., & Kumar, D. (2018). Mentha-Stabilized Silver Nanoparticles for High-Performance Colorimetric Detection of Al(III) in Aqueous Systems. Scientific Reports, 8, 5189. https://doi.org/10.1038/s41598-018-23469-1
- Stack, M., Parikh, D., Wang, H., Wang, L., Xu, M., Zou, J., Cheng, J., & Wang, H. (2019). Chapter 25 - Electrospun Nanofibers for Drug Delivery. En B. Ding, X. Wang & J. Yu (eds.), Electrospinning: nanofabrication and applications (pp. 735-764). William Andrew. https://doi.org/10.1016/B978-0-323-51270-1.00025-X
- Sun, B., Ao, Y. L., Cao, X., Zhang, J. B., Zhang, X., Lu, D. H., Lin, M., Liu, J. S., & Zhang, X. Q. (2025). Hop bitter acids from the pistillate flower of Humulus lupulus L. and their anti-inflammatory and anti-dengue virus activities. Phytochemistry, 241, 114689. https://doi.org/10.1016/j.phytochem.2025.114689
- Xia, Z. K., Ma, Q. H., Li, S. Y., Zhang, D. Q., Cong, L., Tian, Y. L., & Yang, R. Y. (2016). The antifungal effect of silver nanoparticles on Trichosporon asahii. Journal of Microbiology, Immunology and Infection, 49(2), 182-188. https://doi.org/10.1016/j.jmii.2014.04.013
- Xiao, X., Wang, D., Younis, O., Zhang, X., Al-Hossainy, A. F., Yavuz, C. T., Yang, X., & Cheng, H. M. (2025). Recent advances in metallic core-shell nanoparticles for electrocatalysis: synthesis, characterization, and applications. Journal of Energy Chemistry, 110, 227-245. https://doi.org/10.1016/j.jechem.2025.06.057
- Zayed, M. F., Eisa, W. H., El-Kousy, S. M., Mleha, W. K., & Kamal, N. (2019). Ficus retusa-stabilized gold and silver nanoparticles: controlled synthesis, spectroscopic characterization, and sensing properties. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 214, 496-512. https://doi.org/10.1016/j.saa.2019.02.042
- Zehra, S. H., Ramzan, K., Viskelis, J., Viskelis, P., & Balciunaitiene, A. (2025). Advancements in green synthesis of silver-based nanoparticles: antimicrobial and antifungal properties in various films. Nanomaterials, 15(4), 252. https://doi.org/10.3390/nano15040252