Vol. 29 (2019)
Artículos de investigación

Pesticides determination in soil using solid phase extraction and high performance liquid chromatography (HPLC) with diode array detector (DAD)

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  • Roberto Muñiz-Valencia ,
  • José Belisario Leyva-Morales ,
  • José Marcos Jurado Jurado,
  • Orlando Rafael Sarabia-Garcia ,
  • Julia Veronica Hernández Madrigal ,
  • Silvia Guillermina Ceballos-Magaña,
  • Itza Carolina Bejarano Ramirez
Roberto Muñiz-Valencia
http://orcid.org/0000-0002-8678-9289 (unauthenticated) Universidad de Colima

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José Belisario Leyva-Morales
http://orcid.org/0000-0001-6149-7506 (unauthenticated) Universidad Autónoma de Occidente (Unidad Guasave, Sinaloa)

Bio
José Marcos Jurado Jurado Universidad de Sevilla

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Orlando Rafael Sarabia-Garcia
http://orcid.org/0000-0003-3846-6861 (unauthenticated) Universidad de Colima

Bio
Julia Veronica Hernández Madrigal
http://orcid.org/0000-0001-7724-5020 (unauthenticated) Universidad de Colima

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Silvia Guillermina Ceballos-Magaña Universidad de Colima

Bio
Itza Carolina Bejarano Ramirez Universidad de Colima

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Published 2019-11-13

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How to Cite

Pesticides determination in soil using solid phase extraction and high performance liquid chromatography (HPLC) with diode array detector (DAD). (2019). Acta Universitaria, 29, 1-14. https://doi.org/10.15174/au.2019.2287

Abstract

In this work, the development of an analytical method for the determination of carbofuran (CB), diuron (DI), propham (PR), dicloran (DC), chlorpropham (CH), and tetradifon (TE) in soil by high performance liquid chromatography with diode array detector (HPLC-DAD) is presented. For this study, three important optimizations were performed: 1) HPLC-DAD separation; 2) soil sample pretreatment; and 3) solid phase extraction (SPE). Using the optimum chromatographic conditions, the six pesticides were line-base separated in about 17 minutes. The soil sample pretreatment and the SPE were optimized. For both optimizations, a fractional factorial experiment design was applied. The optimum experimental conditions were defined based on the results from the experimental designs. The developed method was validated in soil samples according to international guidelines, evaluating the selectivity, linearity, limit of detection and quantification, as well as accuracy. Finally, the method was applied to other soil samples. The method could be applied in other laboratories to determine these contaminants in soil samples.

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