Identificación de los Genes cry en Cepas Mexicanas de Bacillus thuringiensis con Potencial Insecticida
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Keywords

Bacillus thuringiensis
native strains
cry genes
Cry proteins
PCR.

Abstract

This report revealed the crystal morphology and protein composition of strains LBIT-499 and LBIT-504 of B. thuringiensis, native to Guanajuato, Mexico. LBIT-499 showed only bipyramidal crystals, while LBIT-504 showed both bipyramidal and cubical crystals. These crystals were composed by proteins of ca. 130 and 60 kDa, respectively. Both strains as well as LBIT-500 and LBIT-544, showed a great variety of cry1 genes, while cry2A and cry2B were identified in LBT-500 and LBIT-544, LBIT-504 showed only the cry2B gene, and no cry2 was detected in LBIT-499.

https://doi.org/10.15174/au.2003.268
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