Vol. 34 (2024)
Artículos de Investigación

Optimization and characterization of solvent-tolerant lipases from Geobacillus stearothermophilus CHI1

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  • César Octavio García Hernández,
  • Lucía María Cristina Ventura Canseco,
  • Miguel Abud Archila,
  • Sandy Luz Ovando Chacón,
  • Peggy Elizabeth Alvarez Gutiérrez
César Octavio García Hernández
Instituto Tecnológico de Tuxtla Gutiérrez-Tecnológico Nacional de México
Lucía María Cristina Ventura Canseco
Instituto Tecnológico de Tuxtla Gutiérrez-Tecnológico Nacional de México
Miguel Abud Archila
Instituto Tecnológico de Tuxtla Gutiérrez-Tecnológico Nacional de México
Sandy Luz Ovando Chacón
Instituto Tecnológico de Tuxtla Gutiérrez-Tecnológico Nacional de México
Peggy Elizabeth Alvarez Gutiérrez
CONAHCYT-Instituto Tecnológico de Tuxtla Gutiérrez-Tecnológico Nacional de México
DOI: https://doi.org/10.15174/au.2024.4153

Published 2024-07-24

How to Cite

García Hernández, C. O., Ventura Canseco, L. M. C., Abud Archila, M., Ovando Chacón, S. L. ., & Alvarez Gutiérrez, P. E. (2024). Optimization and characterization of solvent-tolerant lipases from Geobacillus stearothermophilus CHI1. Acta Universitaria, 34, 1–13. https://doi.org/10.15174/au.2024.4153

Abstract

Lipases are enzymes attractive for their use in enzymatic biotechnology, mainly those produced by extremophiles. Recently, the isolation of thermophilic bacteria Geobacillus spp. from the geothermal waters of the crater lake of the “El Chichón” volcano in Mexico was reported. In this work, the lipolytic activity of the G. stearothermophilus CHI1 strain was characterized and optimized. This strain was able to produce enzymes with maximum lipolytic activity at 60 °C and 80°C, with pH values of 5, 9, and 11; showed tolerance of solvents; was able to perform catalysis independently of metal ions; and showed greater affinity towards medium-chain substrates. Results of biochemical characterization and optimization suggest the presence of more than one type of lipolytic activity in Geobacillus stearothermophilus CHI1. All these characteristics make these enzymes attractive in biotechnological processes at high temperatures and alkaline pH (e.g., detergent additives), as well as in the understanding of their biological utility in Geobacillus.

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