Vol. 24 No. 5 (2014)
Artículos de Investigación

Effect of aqueous extracts of leaves of creosote bush (Larreas tridentata), tarbush (Flourensia cernua) and oak (Quercus pungens) on in vitro mycelial growth of phytopathogenic fungi

PDF (Español (España)) HTML (Español (España))
Untitled (Español (España)) Untitled (Español (España)) Untitled (Español (España)) Untitled (Español (España))
  • José Valero Galván,
  • César Alejandro González Díaz,
  • Raquel González Fernández
DOI: https://doi.org/10.15174/au.2014.630

Published 2014-10-24

Keywords

  • Aqueous extracts,
  • fungal pathogens,
  • in vitro control.
  • Extractos acuosos,
  • hongos fitopatógenos,
  • control in vitro.

How to Cite

Galván, J. V., González Díaz, C. A., & González Fernández, R. (2014). Effect of aqueous extracts of leaves of creosote bush (Larreas tridentata), tarbush (Flourensia cernua) and oak (Quercus pungens) on in vitro mycelial growth of phytopathogenic fungi. Acta Universitaria, 24(5), 13–19. https://doi.org/10.15174/au.2014.630

Abstract

In the present study the inhibitory effect of aqueous leaf extracts of creosote bush (Larrea tri­dentata), tarbush (Flourensia cernua) and oak (Quercus pungens) was evaluated on mycelial growth under in vitro conditions of five phytopathogenic fungi (Phytophthora capsici, Botrytis sp., Alternaria solani, Aspergillus flavus and Rhizopus sp.). Aqueous extracts of dried leaves of the three species of plants at two different concentrations (10% and 20%) were performed. Extracts were added to Petri dishes to form a extract-Potato Dextrose Agar (PDA) medium. Subsequently, these medium were inoculated with different pathogens strains and the inhi­bition percentage of mycelium growth of each strain was determined at 24 h, 48 h, 72 h and 96 h. The two concentrations of the extracts of the three plant species showed significant differences in the inhibition percentage of mycelium growth of the various fungi tested. All extracts inhibited the growth of mycelium of P. capsici and A. flavus at 24 h, 48 h and 72 h of incubation at the two concentrations tested. Whereas at 96 h of incubation the F. cernua aqueous extract showed an inhibition percentage of mycelium growth of Botrytis sp. and Rhizopus sp. strains at the two concentrations tested.

PDF (Español (España)) HTML (Español (España))
Untitled (Español (España)) Untitled (Español (España)) Untitled (Español (España)) Untitled (Español (España))

References

  2. Agrios, N. G. (2005). Plant pathology (5 Ed.). San Diego, CA: Elsevier Inc.


  7. Angulo, E. M., Armenta, R. E., García, E. R., Carrillo, F. J., Salazar, V. E. & Valdez, T. J. (2009). Extractos de semilla Swietenia humilis Zucc. con actividad antifungica en Rhizopus stolonifer (Enhernb.:fr.) vuill. Revista Mexicana de Fitopatología, 27(2), 84-92.


  12. Barnett, H. L. (1969). Illustrated Genera of Imperfect Fungi. Department of Plant Pathology, Bacteriology and Entomology. USA: West Virginia University. Morgantown, West Virginia. Minneapolis, Minnesota; Burgess Publishing Company.


  17. Domijan, A., Feraica, M., Jurjevic, Z., Ivil, D. & Cvjetkovic, B. (2005). Fumonisin B1, fumonisin B2, Zearalenone and ochratoxin A contamination of maize in Croatia. Food Additives and Contaminants 22(7), 677-680.


  21. Galvano, F., Piva, A., Ritieni, A. & Galvano, G. (2001). Dietary Strategies to counteract the effect of mycotoxins. A review. Journal of Food Protection, 64(1), 120-131.


  24. Gamboa, A. R., Hernández, C. F., Guerrero, R. E., Sánchez, A. A. & Lira, S. R. (2003). Inhibición del crecimiento micelial de Rhizoctonia solani Kühn y Phytophthora infestans mont (De Bary) con extractos vegetales metanolicos de hojasen (Flourensia cernua D.C). Revista Mexicana de Fitopatología, 21(001), 13-18.


  28. Griffin, G. F. & Chu, F. S. (1983). Toxicity of the Alternaria metabolites alternariol, alternariol methyl ether, altenuene, and tenuazonic acid in the chicken embryo assay. Applied and Environmental Microbiology, 46(6), 1420-1422.


  32. Guerrero, R. E., Solis, G. S., Hernández, C. F., Flores, O. A., Sandoval, L. V. & Jasso, C. D. (2007). Actividad biológica in vitro de extractos de Flourensia cernua D. C. en patógenos de postcosecha: Alternaria alternata (FR.:FR) keissl. Colletotrichum gloeosporioides. Revista Mexicana de Fitopatología, 25(001), 48-53.


  36. Koirala, P., Kumar, S., Yadar, B. K. & Premarajan, K. C. (2005). Occurrence of Aflatoxin in some of the food and feed in Nepal. Indian Journal of Medical Sciences, 59(8), 331-336.


  40. Lamour, K. H., Stam, R., Jupe, J., Huitema E. (2012). The oomycete broad-host-range pathogen Phytophthora capsici. Molecular Plant Pathology 13(4), 329-337


  44. Lira-Saldivar, R. H., Balvantín-García, G. F., Hernández-Castillo, F. D., Gamboa- Alvarado, R., Jasso-De Rodríguez, D. & Jiménez-Díaz, H. (2003). Evaluation of resin content and the antifungal effect of Larrea tridentata (SESEE and MOC. Ex D.C.) Coville extracts from two mexican deserts against Phytium sp. Pringsh. Revista Mexicana de Fitopatología, 21(2), 97-101.


  48. Lopes, M. C. & Martins, V. C. (2008). Fungal plant pathogens in Portugal: Alternaria dauci. Revista Iberoamericana de Micología, 25, 254-256.


  52. López, B. A., López, B. S., Vázquez, B. M., Rodríguez, H. S., Mendoza, E. M. & Padrón, C. E. (2005). Inhibición del crecimiento micelial de Fusarium oxyporum schlechtend. F. SO. lycopersici (Sacc.) Snyder y Hasen, Rhizoctonia solani Kühn y Verticillium dahliae Kleb. mediante extractos vegetales acuosos. Revista Mexicana de Fitopatología, 23(002), 183-190.


  56. Oelke, L., Bosland, P. & Steiner R. (2003). Diferentiation of race specific resistance to Phytophthora Root Rot and Foliar Blight in Capsicum annuum. Journal American Soc Horticultura Science, 128(2), 213-218.


  60. Pose, G., Ludemann, V., Segura, J. & Fernández, P. V. (2004). Mycotoxin production by Alternaria strains isolated from tomatoes affected by Blackmold in Argentina.Mycotoxin Research, 20(2), 80-86.


  64. Rocha, O., Ansari, K. & Doohan, F. M. (2005). Effect of trichothecene mycotoxins on eukaryotic cells: A review. Food Additives and Contaminants, 22(4), 369-378.


  68. Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación (Sagarpa) (2014).Suman esfuerzos para proteger la producción nacional de chile. Noticias. Recuperado el 27 de Mayo del 2014 de http://sagarpa.gob.mx/saladeprensa/2012/Paginas/2014B322.aspx


  72. Singh, H. N. P., Prasad, M. M. & Sinha, K. K (1993). Efficacy of leaf extracts of some medicinal plants against disease development in banana. Apply Microbiology, 17(6), 269-271.


  76. Van West, P., Appiah, A. A. & Gow, N. A. R. (2003). Advances in research on oomycete root pathogens. Physiological and Molecular Plant Pathology, 62(2), 99-113.


  80. Velázquez, D. V., Baños, B. S., Hernández, L. A., Guerra, S. M. & Amora, L. E. (2008). Estrategis de control de Rhizopues stolonifer Ehrenb. (Ex Fr.) lind, agente causal de pudriciones postcosecha en productos agricolas. Revista Mexicana de Fitopatología, 26(001), 49-55.