Vol. 24 (2014): (NE-2) Ciencias Médicas
Artículos de Investigación

Methylation of miR-218-1 in squamous intraepithelial lesions of low grade and cervical cancer with HPV 16

Julio César Muñiz Salgado
Universidad Autónoma de Guerrero
Rubén Barrientos Noriega
Universidad Autónoma de Guerrero
Gabriela Campos Viguri
Universidad Autónoma de Guerrero
Hilda Jiménez Wences
Universidad Autónoma de Guerrero
Dinorah Nashely Martínez Carrillo
Universidad Autónoma de Guerrero
Luz del Carmen Alarcón Romero
Universidad Autónoma de Guerrero
Berenice Illades Aguiar
Universidad Autónoma de Guerrero
Gloria Fernández Tilapa
Universidad Autónoma de Guerrero
José Guadalupe Muñoz Camacho
Instituto Estatal de Cancerología Dr. Arturo Beltrán Ortega
Marco Antonio Jiménez López
Instituto Estatal de Cancerología Dr. Arturo Beltrán Ortega
Víctor Hugo Garzón Barrientos
Instituto Estatal de Cancerología Dr. Arturo Beltrán Ortega
Óscar Peralta Zaragoza
operalta@correo.insp.mx

Published 2015-01-12

Keywords

  • LEIBG,
  • cáncer cervical,
  • miR-218-1,
  • metilación.
  • LGSIL,
  • cervical cancer,
  • miR-218-1,
  • methylation.

How to Cite

Muñiz Salgado, J. C., Barrientos Noriega, R., Campos Viguri, G., Jiménez Wences, H., Martínez Carrillo, D. N., Alarcón Romero, L. del C., Illades Aguiar, B., Fernández Tilapa, G., Muñoz Camacho, J. G., Jiménez López, M. A., Garzón Barrientos, V. H., & Peralta Zaragoza, Óscar. (2015). Methylation of miR-218-1 in squamous intraepithelial lesions of low grade and cervical cancer with HPV 16. Acta Universitaria, 24, 35–38. https://doi.org/10.15174/au.2014.720

Abstract

The alterations in the expression of some microRNAs (miRNAs) in cervical carcinogenesis seems to be related to the aberrant methylation of their promoters. This study evaluates the methylation frequencies of microRNA-218-1 (miR-218-1)’s promoter in low grade squamous intraepithelial lesions (LGSIL) and cervical cancer (CC) with Human Papillomavirus type 16 (HPV-16). In addition,16 samples with LGSIL and 16 with CC were included. The methylation status was performed with qPCR Epitect Methyl II Arrays. The promoter of miR-218-1 was found methylated in 43.75% (7/16) in CC and 6.25% (1/16) in LGSIL (p = 0.037). The promoter of miR-218-1 was found methylated in a higher percentage in CC samples than in LGSIL. It is possible that promoter methylation of miR-218-1 to be an epigenetic mechanism involved in their aberrant expression in CC.