Local levels of inflammatory markers in gingival crevicular fluid in newly diagnosed type 2 diabetics with chronic periodontitis

Abstract

Immune system of diabetic subject is altered due to diabetes mellitus affecting periodontal tissue. This work evaluates inflammatory markers IL-2, IL-6 and IL-8 in gingival crevicular fluid of patients with Type 2 Diabetes Mellitus (T2DM). Participants were grouped into four groups: 1) healthy (n = 20); 2) with periodontitis (PCM, n = 20); 3) ≤1 year with diabetes and periodontitis ((DMT2 ≥ 10+PCM, n = 20); and 4)  ≥ 10 years diabetic and periodontitis (DMT2 ≥ 10 + PCM, n =  20). Cytokines were quantified by flow cytometry. The (DMT2 ≥ 10+PCM group showed higher levels of IL-2 respect to the other groups, as elevated levels of IL-6 respect to the PCM. The levels of IL-8 were higher in the healthy group compared to other groups. (DMT2 ≥ 10 + PCM subjects showed a destructive periodontal tissue adaptive immune response compared to (DMT2 ≥ 1 + PCM which show an immunological disturbance.