This paper describes the use of an ecological alternative to control insect pests. One viable strategy, developed to answer the growing concern about the negative effects that chemical pesticides have on human health and/or the environment, consists in exploiting the use of natural insect pathogens. Among several options, the use of entomopathogenic fungi (EMF) as possible microbial control agents has been considered worldwide. EMF are widely recognized for their potential use as biological control agents. Due to their distinctive mode of action, EMF could have a unique and/or complementary role in insect control. Unlike bacterial, viral or protozoan entomopathogens, EMF do not require to be ingested by their host; instead, germinating fungal spores can penetrate directly through the insect’s cuticle. This mode of infection is possible thanks to the synergistic effect of cuticle-degrading enzymes, plus the mechanic pressure produced upon their contact. The enzymes participating in this process are a determinant factor for an effective infection.