Inhibitory activity of bacteria isolated from the rhizosphere of fruit trees, against Phymatotrichopsis omnivora in vitro

Lorenza Esther Martínez-Escudero, Alicia Melgoza-Castillo, Ramona Pérez-Leal, Elizabeth Villalobos Perez

Abstract


Phymatotrichopsis omnivora affect a great number of fruit species causing great economic losses and the use of agrochemicals to combat them, resulting in adverse environmental effects, so it would be very useful to find alternatives that contribute to reduce the use of these chemicals. There are soil-borne microorganisms with mechanisms of inhibition towards various pathogens, which contribute to the use of biological control methods. The objective of this work was to select and identify bacteria strains capable of inhibiting the growth of P. omnivora. To do this, bacteria were isolated from roots of fruit trees with the presence of the disease to evaluate their antagonistic effect in vitro. A total of 88 bacterial isolates were obtained, of which the antifungal capacity was evaluated by simultaneous confrontation of the fungus and the bacteria in triplicate assays. Cottonseeds were inoculated with strains that showed significant inhibition, using in vitro cultures. The bacterial strains M1, N8 and N15 presented greater biocontrol capacity and, considering the results obtained, they were identified by 16S ribosomal DNA sequencing.


Keywords


Antagonist bacteria; Bacillus subtillis; biological control; molecular biology; rizosphere; cottonseeds.



DOI: https://doi.org/10.15174/au.2018.1606

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Revista Acta Universitaria, volumen 28, núm. 4, julio-agosto 2018, es una publicación bimestral editada por la Universidad de Guanajuato a través de la Dirección de Apoyo a la Investigación y al Posgrado. Lascuráin de Retana no. 5, Centro, C.P. 36000, Guanajuato, Gto., México. Página web: www.actauniversitaria.ugto.mx, correo electrónico: actauniversitaria@gmail.com. Editor responsable: Artemisa Helguera Arellano. Número de Certificado de Reserva de Derechos 04-2013-081513345500-203, ISSN 2007-9621, ambos otorgados por la Dirección de Reservas de Derechos del Instituto Nacional del Derecho de Autor de la Secretaría de Educación Pública. Responsable de la última actualización: Sonia Karina Aguirre Flores. Calzada de Guadalupe s/n, Centro, C. P. 36000, Guanajuato, Gto., México. Fecha de última modificación: 15 de octubre de 2018. Las opiniones expresadas por los autores no necesariamente reflejan la postura del editor de la publicación. Queda estrictamente prohibida la reproducción total sin previa autorización de la Universidad de Guanajuato. Se autoriza la reproducción parcial de los contenidos e imágenes de la publicación Acta Universitaria siempre y cuando se cite la fuente.

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